HIGH-RESOLUTION AUTORADIOGRAPHY

High-resolution Autoradiography

Methods used in obtaining high resolution in autoradiography, with special emphasis on the technique of electron microscopic autoradiography, are described, together with control experiments designed to establish the optimum conditions or procedures. On the basis of these experiments the emulsion selected was Ilford L-4, with a crystal size slightly larger than 0.1 micron. It is applied to the ...

Tritium and Phosphorus-32 in High-Resolution Autoradiography.

The sensitivity of monolayers of Ilford L-4 nuclear emulsion to beta(-)-particles is approximately 0.20 grain per particle emitted for H(3), and 0.025 grain per particle for p(32). The sensitivity for thick layers is 1.3 grains per tritium beta(-)-particle emitted within the emulsion. In electron microscopic autoradiographs the maximum resolution is approximately 0.1 micron for H(3) and 0.3 mic...

High-resolution autoradiography of drugs in fixed tissues.

Mechanisms of transcription in nucleoli of amphibian oocytes as visualized by high-resolution autoradiography

In oocytes of Pleurodeles waltlii, the method of Miller and Beatty has been combined with a method of high-resolution autoradiography especially suitable for the study of isolated molecules. In vitro labeling of RNA by tritiated precursors was carried out with increasing incubation times (1, 4, 15, 24, 48, and 72 h). Silver grains were present over ribonucleoprotein fibrils in amounts sufficien...

Scintillator distribution in high-speed autoradiography.

Durie and Salmon (1) describe the technique of impregnation ofphotographic emulsion with liquid scintillator which allows rapid autoradiographic labeling. In attempting to use this method, we found that freezing the impregnated slides without special conditions led to the formation of large ice crystals and irregularities in the frozen scintillator. They appeared even when the dioxane did not c...